Specific antibodies induced by inactivated parapoxvirus ovis potently enhance oxidative burst in canine blood polymorphonuclear leukocytes and monocytes

Flow cytometric assay for the evaluation of phagocytosis and oxidative burst of polymorphonuclear leukocytes and monocytes in myelodysplastic disorders.

BACKGROUND Myelodysplastic syndromes are clonal diseases characterized by pancytopenia of variable degree. Neutropenia is common and several morphologic and functional abnormalities of polymorphonuclear neutrophilic granulocytes (PMNs) and/or monocytes have been described. On the basis of these observations, the phagocytic and oxygen intermediates production of PMNs and monocytes was determined...

Cytokine Secretion in Monocytes and Peripheral Immune Cells: Induction of Parapoxvirus Ovis (Orf Virus) on Human Immunomodulatory Effects of Inactivated

Polymorphonuclear leukocytes induced vasoconstriction in isolated canine coronary arteries.

To assess how polymorphonuclear leukocytes (PMNs) act on coronary vasoactivity, we measured the changes in isometric tension of isolated rings of canine coronary arteries upon addition of autologous PMNs to organ chambers in which the rings were suspended. When PMNs isolated by the colloidal polyvinylpyrrolidone-coated silica (Percoll) gradient method were added to the chambers, ring preparatio...

Inactivated parapoxvirus ovis (Orf virus) has antiviral activity against hepatitis B virus and herpes simplex virus.

It is known that some viruses are able to induce vigorous immune reactions. This study shows that inactivated parapoxvirus ovis (Orf virus), strain D1701 (PPVO), induces an autoregulatory cytokine response that involves the upregulation of IL-12, IL-18, IFN-gamma and other T helper 1-type cytokines and their subsequent downregulation, which is accompanied by induction of IL-4. An increase in IL...

Independent stimulation of membrane potential changes and the oxidative metabolic burst in polymorphonuclear leukocytes.

Early events of stimulus-response coupling in polymorphonuclear leukocytes (PMNL) reportedly include membrane depolarization as a necessary antecedent to oxidative responses. However, depolarization by nonspecific means (ionophores) is insufficient to elicit an oxidative burst. This apparent conflict might be related to whether depolarization is due to membrane receptor-mediated stimulation of ...